聯系電話:
15821734033
細胞株名稱:caco-2 人結腸癌細胞
上皮細胞 貼壁生長 DMEM,胎牛血清
類別:細胞株;細胞系;細胞株細胞系;人腫瘤細胞;美國ATCC傳代細胞
注意:此細胞僅供科研使用
一、客戶自備試劑:
1、PBS。
2、Complete growth medium。
3、0.25%(W/V) Trypsin-0.53mM EDTA 。
二、細胞簡介:
Growth Properties:adherent
Organism: Homo sapiens (human)
Morphology: epithelial
Source: Organ: colonDisease: colorectal adenocarcinoma
Age: 72 years adult
Gender: male
Ethnicity: Caucasian
Comments: Upon reaching confluence, the cells express characteristics of enterocytic differentiation. Caco-2 cells express retinoic acid binding protein I and retinol binding protein II 。
Propagation:
Complete growth medium: DMEM+10%FBS+P/S
Temperature: 37.0C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed.Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C.
Subc*tion ratio: A subc*tion ratio of 1:2 to 1:5 is recommendedMedium renewal: 2 to 3 times per week
細胞株、Hs578Bst細胞、ND7/23細胞、bel-7407細胞、EA.hy926細胞、A2780細胞、人肝癌細胞株、RAW264.7細胞、NB4細胞、Sp2/0細胞、KYSE30細胞